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Microsoft word - tegreen health care professionals guide.doc


Tegreen97 is a dietary supplement being sold by Pharmanex to support the immune system in the
presence of pollution, stress, and toxins. This is an educational publication provided to help licensed
health care practitioners understand the science upon which Tegreen97 is based and the mechanism
of action by which Tegreen97 works. This pamphlet should not be used to sell Tegreen97, and it
should be distributed only to licensed health care practitioners.
Tea is one of the world’s most popular beverages, second only to water. The health-promoting
benefits of green tea have been recognized in China for thousands of years. However, it is only
recently that subtle but significant differences have been noted between black and green tea in terms
of deriving therapeutic benefits from tea drinking. Evidence is accumulating that the method of
preparing tea leaves plays a pivotal role in preserving the ingredients that are necessary to create tea
with health benefits, the green tea polyphenols.* Recent scientific evidence supports the hypothesis
that urokinase and free radicals can be influenced by polyphenols, especially epigallocatechin-3-
gallate (EGCG).* This review outlines current information on the chemistry and biological activity
of green tea together with laboratory and epidemiological studies of its potential health benefits. It
also further describes Pharmanex's proprietary decaffeinated Tegreen97, which contains consistent
and high levels of medicinal polyphenols.
Tea is one of the most popular beverages worldwide, with an average daily consumption estimated at
close to one 6 fl oz cup (Asian-size cup) per person,1 although there is a large geographical variation
in consumption rates. While most of the world's tea is consumed as black tea, the commercial
manufacture of black tea robs this beverage of polyphenols. In contrast, green tea is prepared so as
to minimize polyphenol oxidation, thus preserving its polyphenol content and medicinal properties.*
Although green and black tea are derived from the same plant (Camellia sinensis), there are
significant differences in their preparation. Black tea (more common in the western world) is
withered, rolled, fermented and dried for curing. Through this flavor-enhancing fermentation process,
the bioactive polyphenols of the fresh leaf are oxidized, and black tea loses its full complement of
beneficial polyphenols.
Green tea, on the other hand, is prepared from lightly steamed and dried leaves of the tea plant. The
steaming process inactivates the enzymes responsible for oxidation, leaving the polyphenol content
intact and resulting in a product with a chemical composition very similar to that of the fresh leaf.
Tegreen97 is prepared by proprietary methods under conditions of strict quality control to ensure a
high concentration of active polyphenols. A full 97% of the important polyphenols of green tea are
preserved in Tegreen97, making this the preparation of green tea with the highest concentration of
polyphenols available in a dietary supplement.*
TeGreen97 provides 160 mg of green tea catechins per capsule. Analytical methods have recently
improved with the ability to measure individual catechins by HPLC methods. A highly credible
recent USDA study determined by HPLC that one Asian size (6 fl oz) cup of traditionally brewed
*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.
Pharmanex Product Support • 1-888-742-7626 (1-888-PHARMANEX) • (1.5 grams of tea leaves per cup) green tea provides about 22 mg of total catechins, half of which is
EGCg.53 Based on this new finding, one capsule of Tegreen97 is equivalent to approximately seven
Asian-size cups of green tea.* This is a significant finding, because studies demonstrate the daily
consumption of four cups of green tea provides significant benefits to overall health.*
Increasing evidence suggests that the therapeutic effects of green tea are mediated by tea
polyphenols.* The mechanisms of tea polyphenols include antioxidant activity, inhibition of
nitrosation reactions, and inhibition of urokinase activity.*
Antioxidant Activities: Powerful antioxidant properties, equivalent to those of vitamin E and greater
than vitamin C,2 3 4 are conferred by the multiple hydroxyl group of the polyphenols, which are
readily oxidized to give the corresponding O-quinones.* As a result, green tea polyphenols are
effective scavengers of active oxygen species such as a wide variety of biomolecules, primarily DNA
and membrane lipids.5* Since the generation of reactive oxygen species is catalyzed by metal ions,
the metal chelating properties of the tea polyphenols also contribute to reducing the levels of oxygen
free radicals in the cell.
Inhibition of Nitrosation Reactions: Endogenous nitrosation of nitrogenous compounds can lead to
the formation of N-nitroso compounds. Green tea has been shown to inhibit nitrosation in vitro by
reacting with nitrosating species.6 *
Inhibition of Urokinase Activity: Urokinase, a proteolytic enzyme naturally found in the body, may
be inhibited by EGCG in the extracellular matrix.7 *
Pre-Clinical Studies
Numerous published scientific studies using green tea extract, known as Xin Nao Jian in Asia,
suggest that the ingestion of green tea polyphenols provides a very broad spectrum of beneficial
physiological functions related to the promotion of general well-being.* In addition, the proprietary
extract found in Tegreen 97 has been shown to exhibit many of these health-promoting benefits.*
Detailed information on the benefits of green tea has been published in a review paper by Mitscher et
al.4 In large scale epidemiological studies in Asia (totaling more than 100,000 people for study
periods up to 10 years), daily consumption of four or more 6 fl oz cups (Asian-size cups) of a green
tea beverage has been associated with significant overall health maintenance of subjects, even after
adjustments were made for age, smoking, alcohol consumption, and relative body weight.
Recent studies have demonstrated that the polyphenols in green tea, particularly the catechin
component, offer potent antioxidant activity through the scavenging of free radicals.* 49 50 Free
radicals, which are involved in the damage of cellular constituents, including DNA, are often the
result of exposure to dietary, occupational and environmental toxins. Upon ingestion, concentrations
of green tea polyphenols can be easily detected in blood, urine and feces, suggesting that polyphenols
are orally bioavailable (absorbed through the gastrointestinal tract).
Antioxidant Protection
In a recent study at Kansas University, researchers found new evidence confirming that a compound
in a green tea supplement Tegreen97 provides stronger free radical damage protection of the genetic
*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.
Pharmanex Product Support • 1-888-742-7626 (1-888-PHARMANEX) • material in cells (DNA), than the well-known antioxidants vitamins E and C, and the antioxidant compound found in red wine.** (**based on in vitro studies) This yet to be published research is the first of its kind to use the Ames test to determine the effectiveness of green tea (using Tegreen97) as an antioxidant to protect against DNA degradation. Using the Ames test, EGCg was added to DNA samples challenged with peroxide, a recognized powerful free radical. The research found EGCg to be a powerful antioxidant, offering 68% protection from DNA damage. The level of protection of DNA samples in the presence of Tegreen97 was significantly higher when compared with a control group of DNA without Tegreen97. This is the first side-by-side antioxidant study comparing the protective power of epigallocatechin gallate (EGCg) against that of vitamins E and C, red wine, and other teas. Using equivalent concentrations in the study, vitamin E was found to be much less protective against damaged cell growth than EGCg or Tegreen97, while vitamin C showed little protective activity at the same concentration. Specifically, EGCg, as found in Tegreen97, was approximately 100 times more effective than vitamin C, 25 times more effective than vitamin E, and nearly twice as effective as the antioxidant compound in red wine at protecting cells. * Numerous experiments and studies indicate that green tea polyphenols, especially EGCg, may help: • block the formation of some potentially toxic compounds, such as nitrosamines, which are formed from the ingestion of some cured meats* • suppress the activation of free radicals* • inhibit spontaneous and photo-enhanced lipid peroxidation* • increase the activity of natural antioxidant and detoxifying enzymes, e.g., glutathione peroxidase, The association between green tea consumption and cancer incidence was studied in a prospective cohort study of a Japanese population. 51 Imai et al., surveyed 8,552 individuals, over 40 years of age, on their living habits, including daily consumption of green tea. They found a negative association between green tea consumption and cancer incidence, especially among females drinking more than 10 cups a day. This epidemiological study showed that green tea has a potentially preventive effect against DNA damage among humans, but in vivo research is still needed. A recent study evaluated the chemopreventive effect of green tea against cigarette smoke-induced mutations (SCE) in humans. 52 Chemopreventive effects of green tea were examined in 52 clinically healthy male subjects between 20-51 years of age. The frequencies of sister-chromatid exchange (SCE) in mitogen-stimulated peripheral lymphocytes, from blood samples, in each experimental group were determined and statistically analyzed. The analysis found that the frequency of SCE in smokers who consumed green tea (7.94 + 0.31) was comparable to that of non-smokers, implying that green tea can block the cigarette-induced increase in SCE frequency. Skin and Dental Health Although sunscreen should always be used, antioxidant supplementation may offer some protective benefits to the skin from free radical damage and the effects of ultraviolet rays. Among the polyphenols that are antioxidants in green tea, EGCg and ECG show the strongest effect in reducing collagenase activity - an enzyme that breaks down collagen.* This reduced enzyme activity may help promote healthy collagen and elastin integrity.* In part, EGCg and ECG do this by promoting a *These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.
Pharmanex Product Support • 1-888-742-7626 (1-888-PHARMANEX) • realignment of these proteins to a more youthful form.* Supplementing the diet with antioxidants,
such as green tea's polyphenols, may lessen the effects of age-related skin damage.*
Researchers from the Department of Clinical Pathology at the Nihon University School of Dentistry
in Japan investigated the ability of liquid green tea extracts for promoting dental health in laboratory
experiments and animals studies. Green tea inhibits the growth of S. mutans and other bacteria
associated with the normal deposit of unwanted or unhealthy materials that may build up on the
surface of teeth after eating certain foods. While more research is needed, regular consumption of
moderate amounts of green tea (and EGCg) may help keep teeth as healthy as possible.*
Green tea characteristics vary widely according to growing region, altitude, climate and processing
techniques. Pharmanex adheres to the time-honored tradition of using only the finest leaf buds and
young leaves of tea plants cultivated in Zhejiang Province, the number one tea-producing region in
China producing some of the highest quality tea in the world. The proprietary extraction process
Pharmanex employs is designed to preserve the beneficial green tea components by maximizing the
concentration of the naturally occurring polyphenols, while virtually eliminating caffeine.
Tegreen97 provides the most concentrated levels of green tea polyphenols available in a dietary
supplement, and is carefully standardized through advanced technologies and proprietary methods.
(See HPLC chart) Other catechins analyzed: DL-Catechin (DL-C); Epicatechin (EC); Gallocatechin
gallate (GCG). Catechins (especially EGCg) appear to have the greatest capacity to quench free
radicals.* Extract also includes: chlorophyll, lignans, proanthocyanidins and phenolic acids.
The polyphenol content of green tea is not known to be associated with any significant side effects or
toxicity. In a long term toxicity study, an extract solution of Tegreen97ª was intragastrically perfused
into rats at 21, 107 and 214 times the recommended adult daily dose for four months. At the end of
four months, the rats showed no abnormalities in general signs, behavior, food intake, feces, and
weight increases. Pathological examinations at the end of four months showed normal organ weight
and morphology of heart, liver, kidney, spleen, lung, stomach, duodenum, adrenal gland, and testicle
or ovary.
Since Tegreen97 is labeled caffeine-free, it should not produce the stimulant effect caused by the
consumption of caffeine-containing beverages in some people. It is recommended that Tegreen97 be
taken with food to minimize any possibility of gastric discomfort.
There is little quantitative information related to the toxicity of green tea catechins. Evidence
derived from epidemiological data indicate that long-term consumption of 10 or more cups per day is
without adverse effects and may be associated with significant health benefits.4 There is no evidence
of long-term or short-term toxicity of green tea extracts.
No drug interactions specific for green tea polyphenols have been identified. However, EGCg has
been shown to inhibit collagen-induced platelet aggregation, so individuals taking aspirin or other
anticoagulant substances on a daily basis should consult with a physician.
*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.
Pharmanex Product Support • 1-888-742-7626 (1-888-PHARMANEX) • DIRECTIONS FOR USE
As a dietary supplement, take one capsule daily with water and food. The polyphenol content in one
capsule of Tegreen97 is equivalent to approximately seven cups of traditionally brewed green tea…
without the caffeine. Tegreen97 should be taken regularly for optimal health benefits.
Tegreen97 capsules (250 mg each) are supplied in a one-month supply of 30 capsules. Clear gelatin
capsules are USP quality and are designed to disintegrate within 30 minutes after ingestion.
Store in dry, cool place. Avoid excessive heat. Protect from light.
Expiration date and lot code numbers are imprinted on the bottom of the box or bottle.
Keep out of reach of children. If you are pregnant or nursing, please consult a healthcare professional
before taking this product.
Each 250 mg capsule of proprietary Tegreen97 contains a 20:1 extract of green tea leaves (Camellia
sinensis) standardized to minimum of 97% pure polyphenols including ³162 mg of catechins of
which > 95 mg is EGCg. Tegreen97 is caffeine-free (<0.5 mg).
* You are taking aspirin or other anticoagulant substances on a daily basis;
* You are lactating, in view of evidence that green tea may interfere with iron metabolism in
*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.
Pharmanex Product Support • 1-888-742-7626 (1-888-PHARMANEX) • KEY REFERENCES
1. Graham HN. Green tea composition, consumption, and polyphenol chemistry. Prev Med
2. Zhao BL, Li XJ, He RG, Cheng SJ, Xin WJ. Scavenging effect of extracts of green tea and natural
antioxidants on active oxygen radicals. Cell Biophys 1989;14:175-185.
3. Luo M, Kannar K, Wahlqvist ML, O'Brien RC. Inhibition of LDL oxidation by green tea extract.
Lancet 1997;349:360-361.
4. Mitscher LA, Jung M, Shankel D, Dou JH, Steele L, Pillai SP. Chemoprotection: A review of the
potential therapeutic antioxidant properties of green tea (Camellia sinensis) and certain of its
constituents. Medicinal Research Reviews 1997;17(4):327-365.
5. Kehrer JP. Free radicals as mediators of tissue injury and disease. Critical Revs Toxicol
6. Nakamura M, Kawabata T. Effect of Japanese green tea on nitrosamine formation in vitro. J Food
Sci 1981;46:306-307.
7. Jankun J, Keck RW, Skrzpczak-Jankun E, Swiercz R. Inhibitors of urokinase reduce the size of
prostate cancer xenografts in severe combined immunodeficient mice. Cancer Res 1997;57:559-563.
8. Merhav H, Amitai Y, Palti H, et al. Tea drinking and microcytic anemia in infants. Am J Clin
Nutr 1985;41:1210-1213.
9. Yang CS, Wang ZY. Tea and cancer. J Natl Cancer Inst 1993;85:1038-49.
10. Zhao BL, Li XJ, He RG, Cheng SJ, Xin WJ. Scavenging effect of extracts of green tea and
natural antioxidants on active oxygen radicals. Cell Biophys 1989;14:175-85.
11. Xu Y, Chi H. The effect of Chinese tea on the occurrence of esophageal tumors induced by N-
nitrosomethylbenzylamine formed in vivo. Biomed Environ Sci 1990;3:406-12.
12. Stich HF. Teas and tea components as inhibitors of carcinogen formation in model systems and
man. Prev Med 1992;21:377-84.
13. Wang ZY, Das M, Bickers DR, Mukhtar H. Interaction of epicatechins derived from green tea
with rat hepatic cytochrome P-450. Drug Metab Dispos 1988;16:98-103.
14. Chen J. The effects of Chinese tea on the occurrence of esophageal tumors induced by N-
nitrosomethylbenzylamine in rats. Prev Med 1992;21:385-91.
15. Wang ZY, Cheng SJ, Zhou ZC, et al. Antimutagenic activity of green tea polyphenols. Mutat Res
16. Wang ZY, Khan WA, Bickers DR, Mukhtar H. Protection against polycyclic aromatic
hydrocarbon-induced skin tumor initiation in mice by green tea polyphenols. Carcinogenesis
17. Khan SG, Katiyar SK, Agarwal R, Mukhtar H. Enhancement of antioxidant and phase II enzymes
by oral feeding of green tea polyphenols in drinking water to SKH-1 hairless mice: possible role in
cancer chemoprevention. Cancer Res 1992;52:4050-2.
18. Billstrom A, Hartley-Asp B, Lancander I, Batra S, Astedt B. The urokinase inhibitor p-
aminobenzamidine inhibits the growth of a human prostate tumor in SCID mice. Int J Cancer
19. Sparks RL, Pool TB, Smith NK, Cameron IL. Effects of amiloride on growth and intracellular
element content of tumor cells in vivo. Cancer Res 1983;43:73-7.
20. Jankun J, Selman SH, Swiercz R, Skrzypczak-Jankun E. Why drinking green tea could prevent
cancer. Nature 1997;387:561.
21. Mukhtar H, Katiyar SK, Agarwal R. Green tea and skin--anticarcinogenic effects. J Invest
Dermatol 1994;102:3-7.
22. Katiyar SK, Agarwal R, Wang ZY, Bhatia AK, Mukhtar H. (-)-Epigallocatechin-3-gallate in
Camellia sinensis leaves from Himalayan region of Sikkim: Inhibitory effects against biochemical
events and tumor initiation in Sencar mouse skin. Nutr Cancer 1992;18:73-83.
*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.
Pharmanex Product Support • 1-888-742-7626 (1-888-PHARMANEX) • 23. Wang ZY, Hong JY, Huang MT, Reuhl KR, Conney AH, Yang CS. Inhibition of N-nitrosodiethylamine- and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced tumorigenesis in A/J mice by green tea and black tea. Cancer Res 1992;52:1943-7. 24. Xu Y, Ho CT, Amin SG, Han C, Chung FL. Inhibition of tobacco-specific nitrosamine-induced lung tumorigenesis in A/J mice by green tea and its major polyphenol as antioxidants. Cancer Res 1992;52:3875-9. 25. Han C, Xu Y. The effect of Chinese tea on occurrence of esophageal tumor induced by N-nitrosomethylbenzylamine in rats. Biomed Environ Sci 1990;3:35-42. 26. Gao GD, Zhou LF, Qi G, et al. Initial study of antitumorigenesis of green tea: Animal test and flow cytometry. Tumor 1990;10:42-4. 27. Wang ZY, Agarwal R, Khan WA, Mukhtar H. Protection against benzo[a]pyrene- and N-nitrosodiethylamine-induced lung and forestomach tumorigenesis in A/J mice by water extracts of green tea and licorice. Carcinogenesis 1992;13:1491-4. 28. Oguni I, Chen SJ, Lin PZ, et al. Protection against cancer risk by Japanese green tea. Prev Med 1992;21:332. 29. Fujita Y, Yamane T, Tanaka M, et al. Inhibitory effect of (-)-epigallocatechin gallate on carcinogenesis with N-ethyl-N'-nitro-N-nitrosoguanidine in mouse duodenum. Japan J Cancer Res 1989;80:503-5. 30. Yamane T, Hagiwara N, Tateishi M, et al. Inhibition of azoxymethane-induced colon carcinogenesis in rat by green tea polyphenol fraction. Japan J Cancer Res 1991;82:1336-9. 31. Narisawa T, Fukaura Y. A very low dose of green tea polyphenols in drinking water prevents N-methyl-N-nitrosourea-induced colon carcinogenesis in F344 rats. Japan J Cancer Res 1993;84:1007-9. 32. Ito N, Hirose M, Shiral T. Carcinogenicity and modification of carcinogenic response by plant phenols. In: Huang MT, Ho CT, Lee CY, (eds.). Phenolic compounds in foods and health II: Antioxidant and cancer prevention. Washington, DC: American Chemical Society 1992;269-81. 33. Qin GZ. Effects of green tea extract on the development of aflatoxin B1-induced precancerous enzyme-altered hepatocellular foci in rats. Chinese J Prev Med 1991;25:332-4. 34. Chung C-H, Liu T-C. Comparative study on the inhibitory effect of green tea, coffee and levamisole on the hepatocarcinogenic action of diethylnitrosamine. Chinese J Oncology 1991;13:193-5. 35. Nishida H, Omori M, Fukutomi Y, et al. Inhibitory effects of (-)-epigallocatechin gallate on spontaneous hepatoma in C3H-HeNCrj mice and human hepatoma-derived PLC-PRF-5 cells. Japan J Cancer Res 1994;85:221-5. 36. Harada N, Takabayashi F, Oguni I, et al. Anti-promotion effect of green tea extracts on pancreatic cancer in golden hamster induced by N-nitroso-bis-oxopropylamine. International Symposium on Tea Science 1991, Japan. 37. Hirose M, Hoshiya T, Akagi K, Futakuchi M, Ito N. Inhibition of mammary gland carcinogenesis by green tea catechins and other naturally occurring antioxidants in female Sprague-Dawley rats pretreated with 7,12-dimethylbenz[alpha]anthracene. Cancer Lett 1994;83:149-56. 38. Liao S, Umekita Y, Guo J, Kokontis JM, Hiipakka RA. Growth inhibition and regression of human prostate and breast tumors in athymic mice by tea epigallocatechin gallate. Cancer Lett 1995;96:239-43. 39. IARC. Coffee, tea, mate, methylxanthines and methylglyoxal. IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. IARC Monogr Eval Carcinog Risks Hum 1991;51:1-513. 40. Ji BT, Chow W-H, Hsing AW, et al. Green tea consumption and the risk of pancreatic and colorectal cancers. Int J Cancer 1997;70:255-8. *These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.
Pharmanex Product Support • 1-888-742-7626 (1-888-PHARMANEX) • 41. Kono S, Ikeda M, Tokudome S, Kuratsune M. A case-control study of gastric cancer and diet in northern Kyushu, Japan. Japan J Cancer Res 1988;79:1067-74. 42. Kato I, Tominaga S, Matsuura A, Yoshii Y, Shirai M, Kobayashi S. A comparative case-control study of colorectal cancer and adenoma. Japan J Cancer Res 1990;81:1101-8. 43. Gao YT, McLaughlin JK, Blot WJ, Ji BT, Dai Q, Fraumeni JF, Jr. Reduced risk of esophageal cancer associated with green tea consumption. J Natl Cancer Inst 1994;86:855-8. 44. Yu GP, Hsieh CC, Wang LY, Yu SZ, Li XL, Jin TH. Green-tea consumption and risk of stomach cancer: A population-based case-control study in Shanghai, China. Cancer Causes Control 1995;6:532-8. 45. Ji BT, Chow WH, Yang G, et al. The influence of cigarette smoking, alcohol, and green tea consumption on the risk of carcinoma of the cardia and distal stomach in Shanghai, China. Cancer 1996;77:2449-57. 46. Kono S, Sinchi K, Ikeda N, Yanai F, Imanishi K. Physical activity, dietary habits and adenomatous polyps of the sigmoid colon; a study of self-defense officials in Japan. J Clin Epidemiol 1991;44:1255-1264. 47. Steinberg NG, Parthasarathy S, Carew TE, Khoo JC, Witztum JL. Beyond cholesterol: modifications of low density lipoprotein that increase its atherogenicity. N Engl J Med 1989;320:915-923. 48. The Alpha-Tocopherol Beta-Carotene Cancer Prevention study group. The effect of vitamin E and beta-carotene on the incidence of lung cancer and other cancers in male smokers. N Engl J Med 1994;330:1029-1035. 49. Fujiki H, Suganuma M, et al. Cancer inhibition by green tea. Mutation Research 1998;402:307-310. 50. Sadzuka Y, Sugiyama T, Hirota S. Modulation of chemotherapy by green tea. Clinical Cancer Research 1998;4:153-156. 51. Imai K, Litt D, Suga K, Nakachi K. Cancer-preventive effects of drinking green tea among a Japanese population. Preventive Medicine 1997;26:769-775. 52. Lee IP, Kim YH, Kang MH, Roberts C, Shim JS, Roh JK. Chemopreventive effect of green tea (Camellia sinensis) against cigarette smoke-induced mutations (SCE) in humans. J Cellular Biochemistry Supplement 1997;27:68-75. 53. Bronner WE, Beecher GR. Method for determining the content of catechins in tea infusions by high-performance liquid chromatography. Journal of Chromatography A 1998;805:137-142. *These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.
Pharmanex Product Support • 1-888-742-7626 (1-888-PHARMANEX) •


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