imported chemical, group-iii

GOVERNMENT OF NCT OF DELHI
MAULANA AZAD MEDICAL COLLEGE
And Associated Lok Nayak, G. B. Pant Hospital & Guru Nanak Eye Centre, (Purchase Branch)
IMPORTED CHEMICAL, GROUP-III
Name of items
Agarose Mol. biology grade for electrophoresis MMLV Reverse transcriptase with H minus activity (10000U) PCR master mix containing Taq DNA polymerase Total nucleic acid Extraction kit/ serum samples(for 100 reactions /kit) HCV RNA quantification Kit ( Taq Man Chemistry 96 tests), primers and probes CEQ DNA separation capillary array 33 cmx 75um Restriction Enzymes Nla4, A1w1, Hph1, Ncil, Earl.
DNA Extraction Kit/solution from serum samples Name of items
Dowex (Fluka) 1x8 chloride form Dry mesh 200-400 Strongly basic anion QIAamp DNA blood mini kit to extract/purify genomic DNA from whole blood, PBMCs, Qx174 DNA molecular marker (H III digest) Sodium hydroxide pellets extra pure for mol. biology Sodium daudisyl suphate (SDS) for molecular biology Ethanol, 100% non-denatured for molecular biology AmpliTaq gold DNA polymerase, 5 units/ul, Supplied with one vial of each 25 mm MgCl2, 10xPCR buffer and 10 m M dNTPs Low molecular weight (50 bp-3 kb) DNA marker PCR master mixture containing Taq DNA polymerase, 2x,with RNase and Acetic acid glacial extrapure for molecular biology Restriction Enzymes, Msp I
Restriction Enzymes, Alu I
Restriction Enzymes, Fok I
Restriction Enzymes, Kpn I
Restriction Enzymes, Taq I
Restriction Enzymes, Dde I
Restriction Enzymes, Bam HI
Name of items
Restriction Enzymes, Glibenclamide
Potassium dihydrogen orthophosate HPLC grade 100. Acid Fuschin101. Alcian blue102. Alpha Naphthyl Acetate anhydrous103. Alpha Naphthyl Acid Phosphate(Monosodium104. Ammonium Persulfate ( Molecular grade)105. Haemotoxylin106. Carmine107. Congo Red108. Crystal violet109. Eosin110. Formamide N N dimethyl111. Haematoxylin crystalline112. Light Green113. TEMED ( Molecular grade)114. Sliver nitrate ( Molecular grade)115. Sodium diethyl barbiturate116. Barbituric Acid117. Naphthol AS phosphate118. Pararosaniline hydrochloride119. Tris buffer pH 7.9 powder120. Amido black Hb electrophoresis slide staining121. Hydrolysed starch122. Nuclear Fast Red123. Neutral red124. Naphthol AS B1 Phosphate125. Fast Blue BB F126. Agarose for gel electrophoresis127. Brilliant Cresyl blue B 5388128. Napthol AS-D Chloracetate129. Fast Garnet GBC130. Basic Fuchsin131. Periodic Acid132. Giemsa Stain133. Nile blue sulfate Name of items
134. Methylene blue135. Ethylene glycol monomethyl ether136. N,N-dimethyl formamide137. Safranin138. Toludine blue139. Azure B140. Dimethyle sulfoxide141. Giemsa stain Fluka142. New Fuchsin143. Wright stain144. Urea145. Boric Acid146. Benzoyl Peroxide147. Methyl Metha crylate148. Dibutyl Phthalate149. NN-Dimethylalamine150. Acrylamide 90% pure DNase,RNase protease free151. TEMED152. TRIZMA base153. Bromophenol blue, sodium salt for molecular biology154. Tri reagent BD155. Proteinase K156. Glycerol157. Xylene cyanol FF158. Ponceau S159. PMSF160. Leupeptin161. Aprotinin162. Triton X-100163. Non fat dried milk164. Tween 20165. Brilliant blue R166. 1,1-Diphenyl-2-Picryl hydrazyl (DPPH)167. 3- (4, 5-Dimethylthiazol2-yl), 2,5-diphenyltetrazolium bromide, AR (MTT).
168. 5,5’-Dithio bis-2-nitrobenzoic acid, AR (Ellman’s reagent/ DTNB)169. Ammonium per sulfate170. Bovine serum Albumin (BSA), fraction V171. Coomassie Brilliant Blue172. Cytochalasin B173. Horse-radish Peroxidase174.
175. Nonidet P-40176. okadaic acid177. Protein A-agarose slurry /bead178. Sodium dodecyl sulfate179. SYBR Green QPCR master mix (2×)180. Sodium-deoxycholate181. 2-Thiobarbituric acid182. Β-mercaptoethanol183. Ficol-hypaque for lymphocyte isolation(hystopaque) Name of items
184. Hydroxyquinone185. 6-OH - 2,5,7,8 Tetra methyl chroman 2 - Carboxylic acid(Trolax) AR186. Triethylamine(MS/MS grade)187. Acetonitrile (MS/MS grade)188. OPA Reagent189. FMOC Reagent190. Amino Acid standard (25pmol)191. Secondary Amino Acid standard (25pmol)192. 0.22µm filter (13mm diameter)193. Tetrahydrofuran (THF-MS/MS grade)194. Silica Gel (230-400 mesh) Merck Grade195. Ethyl Acetate196. 4Methylumbelliferone197. Sodium Phosphate monobasic Anhydrous(Na2H2PO4)198. N- Acetylgalactosamine.
199. Sodium taurocholate200. Chaps201. Sodium Citrate tribasic Dihydrate202. Acarbose203. Oleic Acid204. Saccharolactone205. Phenol TE Saturated206. Methyl Melonic Acid deutrated standard for MS analysis207. Complete Media for chorionicvillus culture208. Fud R209. Thymidene210.
Complete Media for Amniotic Fluid culture 211. Acetonitrile (LCMS/Ms grade)212. Methanol (LCMSMS Grade)213. LCMSMS grade Water214. Tetrahydrofuran HPLC grade215. Triethylamine HPLC grade216. Complete medium for Lymphocyte culture217. Colchimd218. Giemsa stain in GURR’s buffer219. 2XMaster Mix220. Taq Polymérase221. dNTP’S222. Trireagent223. BSTFA + TMCS,99 :1(Sylon BFT )224. Pyridine225. Primers for analysis of the GHRd3 226. PCR primers used for amplification of coding sequences and intron-exon PRIMER SEQUENCE
Forward: ATATGACTCACCTGATTCATGCReverse: TAGGTACATCCATGGAGAGGAA Forward: GCTACAACATGATTTTTGGAACAAReverse: CATTTATTTAGTCTAAAACTATGTCA Forward: AATATTAAATTGTGTCTGTCTGTGTReverse: GAAAGAAAAGTCAAAGTGTAAGGT Forward: AGAATATGTAGCTTTTAAGATGTCAReverse: GACAGGAGTCTTCAGGTGTTAA Forward: GAGTTTCTTTTCATAGATCTTCReverse: TTAACCTCTGTGGCTGAG Forward: ACATGAGGGTACCTCAGAReverse: CAGAAGTAGGCATTGTCC Forward: GGAAATGGTCTCACTCTGReverse: CAAAAGAAAGGCTAAGGC Exon 2 – 112 - 5'AGCTCCTATTCCACCCCTCTTC 3' Exon 2 - 70* - 5'AAAATCTCACCTTAATGTTGTCC 3'Exon 9 -1 - 5'CCTTTCCTTGACTTTTTC 3' Exon 9- 2* - 5'TGCCATTACCCTCTTTTG 3'Exon 11- 1 - 5'ACTTGAAGATTTGCTGTG 3' Exon 11- 2* - 5'TGCTGTCATCATTTTACTAC 3'Exon 12-1 - 5'CAGGGTTTTTATAGTCATTA 3'Exon 12-2* - 5'CAAGATTCCCATCACAGT 3' Thalasemia (Primers 5'<-----Sequence----->3')
CONTROL 1 -- CAA TGT ATC ATG CCT CTT TGC ACC
CONTROL 2 -- GAG TCA AGG CTG AGA TGC AGG ACOMMON A -- ACC TCA CCC TGT GGA GCC ACCOMMON B -- CCC CTT CCT ATG ACA TGA ACT TAA Fr.41/42 M -- GAG TGG ACA GAT CCC CAA AGG ACT CAA CCTFr.41/42 N -- GAG TGG ACA GAT CCC CAA AGG ACT CAA AGA IVSI nt 5 F M -- CTC CTT AAA CCT GTC TTG TAA CCT TGT TAGIVSI nt 5 N -- CTC CTT AAA CCT GTC TTG TAA CCT TGT TAC IVSI nt 1 M -- TTA AAC CTG TCT TGT AAC CTT GAT ACG AAAIVSI nt 1 N -- GAT GAA GTT GGT GGT GAG GCC CTG GGT AGG Fr.8/9 M -- CCT TGC CCC ACA GGG CAG TAA CGG CAC ACC
Fr.8/9 N -- CCT TGC CCC ACA GGG CAG TAA CGG CAG ACT
Renalase
rs2296545 F -- 5' GGA AGT CCC CGA TCA CGT GAC 3'
rs2296545 R -- 5' TGC TGT GTG GGA CAA GGC TGA 3'rs2576178 F -- 5'AGC AGA GAA GCA GCT TAA CCT 3' rs2576178 R -- 5'TTA TCT GCA AGT CAG CGT AAC 3'
MTHFR
MTHFR 677TR -- CCC ATG TCG GTC CAT CCC TT
MTHFR1298A-C F - CAAGGAG-GAGCTGCTGAAGA
MTHFR1298A-CR - CCACTCCAG-CATCACTCACT
G6PD
G6PD Primers nt 563 (C-T) 330 bp

Source: http://www.mamc.ac.in/pdf/imported_chemical.pdf